[PLAGUES] COVID-19, H5N1, probably demodex eventually

[Falcon Analyst]

CDC A(H5N1) BIRD FLU UPDATE

https://www.cdc.gov/bird-flu/spotlights ... 82024.html

[quote]Continuing to work with public health laboratories to monitor influenza virus testing. While 53 people have been tested for A(H5N1) virus associated with the dairy cow outbreak, it is important to look at exposure-based testing in the context of CDC's wider testing for flu in the United States. Since March 3, 2024, 31,223 specimens have been tested by public health laboratories for flu with results being reported to CDC. These were specimens submitted to public health laboratories for flu testing and subtyping as part of regular flu surveillance activities. Public health laboratories report data to CDC each week, and more than 200,000 specimens are tested each year. These laboratories use a testing protocol that would detect A(H5N1) virus and other novel viruses. Among those more than 30,000 specimens tested since March 3, 2024, no cases of H5N1 bird flu were detected.

The Michigan Department of Health and Human Services (MDHHS) launched an H5N1 bird flu seroprevalence study of individuals exposed to sick cows. The study aims to determine if there has been asymptomatic infection with H5N1 bird flu among people who have worked with sick cows, if certain jobs might increase risk of exposure, and how personal protective equipment (PPE) can protect against infection. CDC is providing technical assistance

[/quote]

[UAE Bird Expert]

"Evidences of neurological injury caused by COVID-19 from glioma tissues and glioma organoids"
https://onlinelibrary.wiley.com/doi/ful ... /cns.14822

Abstract
Introduction
Despite the extensive neurological symptoms induced by COVID-19 and the identification of SARS-CoV-2 in post-mortem brain samples from COVID-19 patients months after death, the precise mechanisms of SARS-CoV-2 invasion into the central nervous system remain unclear due to the lack of research models.

Methods
We collected glioma tissue samples from glioma patients who had a recent history of COVID-19 and examined the presence of the SARS-CoV-2 spike protein. Subsequently, spatial transcriptomic analyses were conducted on normal brain tissues, glioma tissues, and glioma tissues from glioma patients with recent COVID-19 history. Additionally, single-cell sequencing data from both glioma tissues and glioma organoids were collected and analyzed. Glioma organoids were utilized to evaluate the efficacy of potential COVID-19 blocking agents.

Results
Glioma tissues from glioma patients with recent COVID-19 history exhibited the presence of the SARS-CoV-2 spike protein. Differences between glioma tissues from glioma patients who had a recent history of COVID-19 and healthy brain tissues primarily manifested in neuronal cells. Notably, neuronal cells within glioma tissues of COVID-19 history demonstrated heightened susceptibility to Alzheimer's disease, depression, and synaptic dysfunction, indicative of neuronal aberrations. Expressions of SARS-CoV-2 entry factors were confirmed in both glioma tissues and glioma organoids. Moreover, glioma organoids were susceptible to pseudo-SARS-CoV-2 infection and the infections could be partly blocked by the potential COVID-19 drugs.

Conclusions
Gliomas had inherent traits that render them susceptible to SARS-CoV-2 infection, leading to their representability of COVID-19 neurological symptoms. This established a biological foundation for the rationality and feasibility of utilization of glioma organoids as research and blocking drug testing model in SARS-CoV-2 infection within the central nervous system.
(..)
Whether the SARS-CoV-2 can infect the brain tissue directly remains a subject of uncertainty. The ambiguity arises primarily due to the scarcity of brain tissues for research proposes, particularly specimens that have been recently infected with SARS-CoV-2. To address this challenge, we adopted an alternative approach by collecting surgically removed glioma tissue samples from glioma patients who had recently undergone COVID-19, aiming to elucidate the conditions and mechanisms underlying the SARS-CoV-2 invasion of the brain.

A 44-year-old male patient who had received three doses of the COVID-19 vaccine presented with glioblastoma in the left parietal lobe. Tumor resection surgery was performed 51 days after COVID-19 recovery. The patient's primary complaint during COVID-19 illness was mild body soreness lasting for 4 days. Immunofluorescence analysis revealed the presence of SARS-CoV-2 spike protein and ACE2 receptor expression in the resected glioma tissue (Figure 1A). In another case, a 31-year-old female patient who had received two doses of the COVID-19 vaccine reported symptoms including fever, general pain, weakness, dizziness, headache, and loss of taste and smell during COVID-19 illness. Seventy-four days after contracting COVID-19, surgical resection was conducted to remove an astrocytoma (IDH-mutant, CNS WHO grade 4) in the right frontal lobe. Immunofluorescence analysis demonstrated the presence of SARS-CoV-2 spike protein and ACE2 receptor expression in the resected glioma tissue (Figure 1B).
(..)
The included 10 samples were integrated (after de-batching process) to illustrate their correlations and differences (Figure 2B,C). Remarkably, whether in glioma tissues or glioma-COVID tissues, certain clusters of cells exhibited a level of health comparable to that observed in normal brain tissues. Meanwhile, a substantial number of cells in both the glioma tissue group and glioma-COVID tissue group displayed distinct characteristics when compared with cells in normal brain tissues.

The distinct genes (differentially expressed genes, DEGs) expressed in glioma-COVID tissues were annotated with KEGG pathway analysis. As anticipated, the most pronounced pathways enriched in glioma-COVID tissues comparing with normal brain tissues (Figure 2D) or glioma tissues (Figure 2E) were both the COVID-19-related pathway.

We isolated neuronal cells from each sample based on neuron markers (Figure S1), in conjunction with NPC2 scoring (Figure S2), and subsequently examined the distinctions among neuronal cells derived from the three different tissue types. Notably, neuronal cells derived from glioma-COVID tissues exhibited a distinct separation from those from normal brain tissues (Figure 3A,B). Interestingly, in contrast to neuronal cells, non-neuronal cells (i.e., cells other than neuronal cells) appeared to be less influenced by COVID-19. A considerable number of non-neuronal cells in glioma-COVID tissues maintained a phenotype similar to that observed in non-neuronal cells from normal brain tissues (Figure 3C,D).
(..)

[UAE Bird Expert]

On July 6, from 6:45 am to 6:00 pm Pacific time, PhysicsGirl is going to livestream a day in her life as a fundraiser for long COVID and ME/CFS research: http://youtube.com/post/UgkxsWqCFbvcTSA ... 7ZSGuAQ8pp

Will have Q&A time with her and expert interviews too.

[UAE Bird Expert]

BREAKING: The U.S. will pay vaccine maker Moderna $176 million to develop a pandemic vaccine that could be used to treat bird flu in people.

[UAE Bird Expert]

[UAE Bird Expert]

You did it Joe

[UAE Bird Expert]

https://www.news.com.au/lifestyle/healt ... 06e6870d38

‘Top five’: Shocking Covid stat as cases soar
A chief health officer says three new varients of Covid are spreading in Australia, as new statistics about the disease come to light.

Experts have sounded a warning over “complex” and highly-transmissible Covid subvariants taking off in Australia — as new data shows the virus is among the nation’s top five killers.

Queensland chief health officer John Gerrard told the Courier Mail three sub-variants are now spreading in the state – FLiRT, FLuQE and most recently de-FLiRT.

“Due to this mutation, as well as fluctuating community immunity, we are witnessing more than one wave each year,” he said. “Covid-19 has not yet developed a clear seasonal pattern.”

The FLuQE subvariant is ripping through the US will likely produce a bulge in new cases in Australia, experts have warned.

This comes as newly-published data shows the illness has become the third leading cause of death in Australia — marking the first time in over fifty years an infectious disease has ranked so highly in the nation’s mortality statistics.

According to the Australian Institute of Health and Welfare (AIHW), with 9,859 deaths attributed to the virus, Covid-19 trailed only coronary heart disease and dementia, including Alzheimer’s disease, as a leading cause of death.

The AIHW’s “What do Australians Die From” report released this week provides a comprehensive overview of the health conditions involved in the 191,000 registered deaths in Australia in 2022.
(..)

[UAE Bird Expert]

Oh fuck, live virus is lurking in bone tissue


https://wwwnc.cdc.gov/eid/article/30/8/24-0145_article

Our findings revealed that SARS-CoV-2 was detectable by PCR in 9.1% and by viral culture in 4.5% of skull sawdust samples, suggesting the presence of live virus and a risk, although low, of infective viruses becoming aerosolized. We could not identify previous work examining cranial sawdust for the presence of pathogens, but our results align with a previous study showing SARS-CoV-2 PCR positivity for 4.5% of goggles and no masks tested after autopsy (7).

The sample size for our study was limited but represents a consecutive and nonselected series of cases at a single institution. We did not directly assess aerosols, but given that bone sawing is the only high-energy technique used, and considering the findings from a previous study (7), the presence of concomitant other sources of infective aerosols in the autopsy room is unlikely. The personnel present during COVID-19 autopsies were not systematically tested, but symptomatic persons were extensively PCR tested for SARS-CoV-2 during the study period (2020–2022). In addition, skull sawdust samples might not consist solely of bone and could contain adjacent tissues because of anatomy, particularly the frontal sinus, which is lined with respiratory epithelium. Skullcap sawing has the potential to generate infective aerosols, but in our experience, general autopsy safety measures are effective. The absence of positive findings in our CNS samples give confidence in the sterility of our sampling technique, thereby making other sources of contamination in the skull sawdust samples less likely.

[Diplotomodon]

Oh fuck, live virus is lurking in bone tissue


https://wwwnc.cdc.gov/eid/article/30/8/24-0145_article

Our findings revealed that SARS-CoV-2 was detectable by PCR in 9.1% and by viral culture in 4.5% of skull sawdust samples, suggesting the presence of live virus and a risk, although low, of infective viruses becoming aerosolized. We could not identify previous work examining cranial sawdust for the presence of pathogens, but our results align with a previous study showing SARS-CoV-2 PCR positivity for 4.5% of goggles and no masks tested after autopsy (7).

The sample size for our study was limited but represents a consecutive and nonselected series of cases at a single institution. We did not directly assess aerosols, but given that bone sawing is the only high-energy technique used, and considering the findings from a previous study (7), the presence of concomitant other sources of infective aerosols in the autopsy room is unlikely. The personnel present during COVID-19 autopsies were not systematically tested, but symptomatic persons were extensively PCR tested for SARS-CoV-2 during the study period (2020–2022). In addition, skull sawdust samples might not consist solely of bone and could contain adjacent tissues because of anatomy, particularly the frontal sinus, which is lined with respiratory epithelium. Skullcap sawing has the potential to generate infective aerosols, but in our experience, general autopsy safety measures are effective. The absence of positive findings in our CNS samples give confidence in the sterility of our sampling technique, thereby making other sources of contamination in the skull sawdust samples less likely.

uh oh

[consentvan]

Went to a pool party a couple weeks ago, stayed well away from everyone and always upwind, it’s Texas so I brought a umbrella to assist so that clinging to shade didn’t put me in a bad spot. But the food was inside so I would put on my p100 respirator to get a plate, later a lady asked “what’s with the gas mask?” And was astonished to hear I hadn’t had Covid, much less been sick period in five years.

LOL she just got Covid again, I hope she remembers being an ass.

This surge in particular has been real good for things like that to make me feel a little less insane that we’re still doing this. My partners work friend and her husband were the only people we knew irl who still mask and we did a couple outside hang out sessions. Well they stopped being serious about precautions and got their first Covid infection this last week.

Every new surge it’s people who fell for the gaslighting dropping precautions and getting their Covid cherry popped.

There’s so few of us now.